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laboratory_safety_regulations.docx
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micro_techniques_lab.docx
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lab_1_light_microscopynew.docx
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lab_2_aseptic_technique.docx
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lab_3_negative_and_simple_staining.docx
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lab_4_gram_stainnew.docx
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lab_5_ubiquitous_nature_of_microorganisms_revised.docx
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lab_6_controlling_microbes.docx
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lab_7_medialab.docx
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lab_8_1001_dna_gel_electrophoresis.pdf
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lab_9_6b_restriction_digest.pdf
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lab_10_1004_genotype_to_phenotype.pdf
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lab_11_plasmid_isolation_fall_2019.doc
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bonus_lab_prepared_specimes.pdf
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Sample Restriction Mapping Problem

Digestion of a 4 kb DNA molecule with EcoRI yields two fragments of 1
kb and 3 kb each. Digestion of the same molecule with HindIII yields
fragments of 1.5 kb and 2.5 kb. Finally, digestion with EcoRI and
HindIII in combination yields fragments of 0.5 kb, 1 kb, and 2.5 kb.
Draw a restriction map indicating the positions of the EcoRI and
HindIII cleavage sites.
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