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Sample Restriction Mapping Problem
Digestion of a 4 kb DNA molecule with EcoRI yields two fragments of 1
kb and 3 kb each. Digestion of the same molecule with HindIII yields
fragments of 1.5 kb and 2.5 kb. Finally, digestion with EcoRI and
HindIII in combination yields fragments of 0.5 kb, 1 kb, and 2.5 kb.
Draw a restriction map indicating the positions of the EcoRI and
HindIII cleavage sites.
Digestion of a 4 kb DNA molecule with EcoRI yields two fragments of 1
kb and 3 kb each. Digestion of the same molecule with HindIII yields
fragments of 1.5 kb and 2.5 kb. Finally, digestion with EcoRI and
HindIII in combination yields fragments of 0.5 kb, 1 kb, and 2.5 kb.
Draw a restriction map indicating the positions of the EcoRI and
HindIII cleavage sites.