Sample Restriction Mapping Problem
Digestion of a 4 kb DNA molecule with EcoRI
yields two fragments of 1 kb and 3 kb each. Digestion of the same molecule with HindIII yields fragments of 1.5 kb and
2.5 kb. Finally, digestion with EcoRI and HindIII
in combination yields fragments of 0.5 kb, 1 kb, and 2.5 kb.
Draw a restriction map indicating the positions of the EcoRI
and HindIII cleavage sites.
yields two fragments of 1 kb and 3 kb each. Digestion of the same molecule with HindIII yields fragments of 1.5 kb and
2.5 kb. Finally, digestion with EcoRI and HindIII
in combination yields fragments of 0.5 kb, 1 kb, and 2.5 kb.
Draw a restriction map indicating the positions of the EcoRI
and HindIII cleavage sites.